High Throughput/Content Screening Facility of the CIDD

High Throughput Screening Process:

Performing HTS can be broken down into 4 phases:

  1. Assay design
  2. Assay optimization
  3. Primary assay screening
  4. Hit confirmation

The design of the primary screening assay (including formatting and optimization) is a crucial step in HTS that will determine the biological relevance of the compounds identified. Thus, given a defined biological question, the primary assay should be carefully designed and developed.

The HTS Core at UTHSCSA can provide investigators with the equipment, chemical libraries and know-how for performing both biochemical and cell-based screens using a variety of detection formats. The HTS Core's mission is to provide cost-effective and time-efficient access to HTS technology to all researchers in the San Antonio area, from singling out a precise biological process to be targeted for interrogation, to the analysis of HTS data and the identification and validation of hits.

Services provided by the CIDD HTS facility:

  • Consultation on the design of high throughput assays (this is a crucial step in HTS that will determine the biological relevance of the compounds identified)
  • Biological/Biochemical assay optimization.
  • Development, testing and screening of libraries using cell-based high-content imaging, cell-based conventional or biochemical-based screens.
  • Cherry-picking of potential lead compounds.
  • Development of secondary assays and counter-screens.
  • Identification of lead compound drug candidates.

Lead compound candidates are then transferred to the CIDD Medicinal Chemistry Core at UTSA for medicinal chemistry development and optimization (Stanton F. McHardy, Stanton.McHardy@utsa.edu).